ABSTRACT
Plants, especially medicinal plants, took up the most space during the production of silver nanoparticles and have shown significant promise for use in biotechnology. So the goal of this research was to focus on a type of lung cell line, the WI-38 cell line, infected with the Corona virus. The study also included the isolation of the active compounds from the roots of the Taraxacum officinale plant, their extraction, and the study of their biological effects. Used in this study were: Taraxacum officinale extract, silver nitrate, (Dulbecco's Modified Eagle Medium (DMEM), fetal bovine serum (FBS) L-glutamine, penicillin, streptomycin, and dimethyl sulfoxide (DMSO) from Sigma Aldrich, USA), and XTT (2,3-bis(2-methoxy-4-nitro-5-sulfo phenyl)-2H-tetrazolium5-carboxamide) from RIbobio-China. WI-38 CCL75 cell lines were purchased from American Type Culture Collection (ATCC) identification. Source was the lung tissue for female-3 month gestation and the SARS-CoV-2 virus. Oils, flavonoids, glycosides, and tannins from Taraxacum officinale were extracted in the extraction device (Soxhlet) in successive stages using several solvents. Silver nanoparticles Ag NPs were synthesized using the green method from these extracts and diagnosed by X-Ray diffraction analysis XRD, Fourier transform infrared spectroscopy (FTIR), and field emission scanning electron microscopy (FESEM). Cell cultures were used to study the effect of nanoparticles on lung cells infected with the Coronavirus (SARS-CoV-2) using lung cell lines (wi-38) . The activity of these particles as antivirals was evaluated due to their antiviral activity where pure cultures of cell lines were planted on DMEM where DEME was used as a positive control and the cell line with DMEM was a negative control. For each experiment, the diameter of the inhibition area was measured in millimeters. Finally, the XTT test was used to test the extracted and the nanoparticle solution to ensure its suitability for inhibition of the virus coronavirus (SARS-COV-2). The results showed that nanoparticles have strong antiviral efficacy against the coronavirus. 50 mg/L of the Ag NPs extract was found to have the greatest inhibition. The potent bioactivity of the synthetic green silver nanoparticles derived from medicinal plants supports their biological use as an antiviral. The study also showed the effects of different concentrations of silver nanoparticle solutions on cell growth. The presence of phenolics and flavonoids was found in the alcoholic and aqueous extracts of the T. officinale roots. The mechanism of action of Ag NPs was investigated. As noted, the Ag NPs alcoholic extract outperformed the Ag NPs aqueous extract in terms of growth because of its small size. From this study, we conclude that the method of green biosynthesis of metal nanoparticles is considered safe and inexpensive, and the materials produced are not contaminated. Nanoparticles can be applied in many applications depending on their physical properties, such as the size and shape of the particle. They were also tested in vitro against coronavirus (SARS-COV-2) utilizing the cell line. Interestingly, the antiviral activities of Ag NPs alcoholic and aqueous extracts against SARS-CoV-2 were noteworthy, with IC50 values of 32.50 and 29.03, respectively. The findings might be a suitable starting point for future optimization and more sophisticated preclinical and clinical research of molecules on single components, particularly alcoholic extract, for inhibiting and lowering the activity of the Coronavirus in infected cells. © 2023, Consulting and Training Center - KEY. All rights reserved.
ABSTRACT
Symptoms of COVID-19 vary and range from mild symptoms to serious illness. Common symptoms include cough, fever, loss of smell and tastes. The aim of this study is to measure some biochemical parameter in covid-19 patients 2 weeks after recovery.. Method: The study included 50 patients suffering from infection with the emerging corona virus and 30 samples of healthy people as a control group, and both groups ranged in age from 27-77 years and of both sexes, collected from Kirkuk General Hospital. A number of biochemical variables were measured in the blood serum of the groups under study, including blood urea and creatinine, two weeks after infection. The study revealed that the risk of infection with the Covid-19 virus increases with age, the most affected are the elderly, as the study found that 48% of patients were older than 55 years of age and 15% within the age group 41-55 years. The study also found that males were more affected by the virus than females (52% males and 48% females.